The Molecular Bioelectricity Lab Jianmin Cui, Principal Investigator

On BK-type Ca2+ activated K+ channels

22. Zhang G, Geng Y, Jin Y, Shi J, McFarland K, Magleby KL, Salkoff L, Cui J. (2017) Deletion of cytosolic gating ring decreases gate and voltage sensor coupling in BK channels. J Gen Physiol 149(3):373-387. doi: 10.1085/jgp.201611646. PMID: 28196879(Download)

21. Zhang G, Yang H, Liang H, Yang J, Shi J, McFarland K, Chen Y, Cui J. (2014) A charged residue in S4 regulates coupling among the activation gate, voltage, and Ca2+ sensors in BK channels. J Neurosci. 34(37):12280-8. doi: 10.1523/JNEUROSCI.1174-14.2014. PMID: 25209270 (Download)

20. Li M, Chang S, Yang L, Shi J, McFarland K, Yang X, Moller A, Wang C, Zou X, Chi C, Cui J. (2014) Conopeptide Vt3.1 preferentially inhibits BK potassium channels containing β4 subunits via electrostatic interactions. J Biol Chem. 289(8):4735-42. doi: 10.1074/jbc .M113.535898 PMID: 24398688 (Download)

19. Sun X, Shi J, Delaloye K, Yang X, Yang H, Zhang G, and Cui J (2013) The interface between membrane- spanning and cytosolic domains in BK channels is involved in β subunits modulation of gating. J Neurosci. 33:11253-11261. doi: 10.1523/JNEUROSCI.0620-13.2013. PMID: 23825428 (Download)

18. Yang, J., Delaloye, K., Yang, X., Moller, A., Shi, J. and Cui, J. (2013) Interactions between structural domains during BK channel gating. J. Gen. Physiol. 141:217-228. doi:10.1085/jgp.201210794 . PMID: 23359284 (Download)

17. Deng, P.Y., Rotman, Z., Blundon, J.A., Cho Y., Cui, J., Cavalli, V.M., Zakharenko, S.S., and Klyachko, V.A. (2012) FMRP Regulates Neurotransmitter Release and Synaptic Information Transmission by Modulating Action Potential Duration via BK channels. Neuron 77:696-711. PMCID: 3584349 (Download)

16. Yang, J., Delaloye, K., Lee, U.S., and Cui, J. (2011) Patch clamp and perfusion techniques for studying ion channels expressed in Xenopus oocytes. J Vis Exp. 47, pii: 2269. doi: 10.3791/2269. PMID: 21248703 (Link)

15. Zhang, G., Huang, S.Y., Yang, J. Shi, J., Yang, X., Moller, A., Zou, X., and Cui, J. (2010) Ion sensing in the RCK1 domain of BK channels. Proc. Natl. Acad. Sci., U.S.A. 107:18700-18705. PMCID: 2972974. (Download)

14. Lee, U.S., Shi, J. and Cui, J. (2010) Modulation of BK channel gating by the β2 subunit involves both membrane-spanning and cytoplasmic domains of Slo1. J Neurosci. 30:16170-16179. PMCID: 3073600. (Download)

13. Yang, J,, Krishnamoorthy, G., Saxena, A., Zhang, G., Shi, J., Yang, H., Delaloye, K., Sept, D., and Cui, J. (2010) An epilepsy/dyskinesia-associated mutation enhances BK channel activation by potentiating the Ca2+ dependent allosteric mechanism. Neuron 66:871-883. PMCID: 2907746 [Previews: Neuron 66, 817- 818]. (Download)

12. Lee, U.S. and Cui, J. (2009) β subunit-specific modulations of a mutant BK channel associated with epilepsy and dyskinesia. J. Physiol. (London) 587:1481-1498. PMCID: 2678220 (Download)

11. Yang, H., Shi, J., Zhang, G., Yang, J, Delaloye, K., and Cui, J. (2008) Activation of Slo1 BK channels by Mg2+ coordinated between the voltage sensor and the RCK1 domains Nature Structure and Molecular Biology 15:1152-1159. PMCID: 2579968 [News & Views: Nature Structure and Molecular Biology 15:1130 -1132]. (Download)

10. Yang, H., Zhang, G., Shi, J., Lee, U.S., Delaloye, K., and Cui, J. (2008) Subunit-specific Effect of the Voltage Sensor Domain on Ca2+ Sensitivity of BK Channels. Biophys. J., 96:4678-4687. PMCID: 2397326 (Download)

9. Yang, H., Hu, L., Shi, J., Delaloye, K., Horrigan, F., and Cui, J. (2007) Mg2+ Mediates Interaction between the Voltage-Sensor and Cytosolic Domain to Activate BK channels. Proc. Natl. Acad. Sci., U.S.A. 104, 18270-18275. PMCID: 2084332 [Commentary: J. Gen. Physiol. 131, 5-11] (Download)

8. Yang, H., Hu, L., Shi, J., and Cui, J. (2006) Tuning magnesium sensitivity of BK channels by mutations. Biophys. J., 91, 2892-2900. PMCID: 1578465 (Download)

7. Lei Hu, Huanghe Yang, Jingyi Shi, and Jianmin Cui (2006) Effects of multiple metal binding sites on calcium and magnesium-dependent activation of BK channels. J. Gen. Physiol. 127, 35-49. PMCID: 2151482 (Download)

6. Krishnamoorthy, G., Shi, J., Sept, D., and Cui, J. (2005) The N-terminus of RCK1 domain regulates Ca2+ dependent BKCa channel gating. J. Gen. Physiol. 126, 227-241. PMCID: 2266574 (Download)

5. Du, W., Bautista, J. F., Yang, H., Diez-Sampedro, A., You, S.-A., Wang, L., Kotagal, P., Lüders, H. O., Shi, J., Cui, J., Richerson, G. B., and Wang, Q. (2005) Calcium-sensitive potassium channelopathy in human epilepsy and paroxysmal movement disorder. Nat. Genet. 37, 733-738 PMID: 15937479 (Download)

4. Guoxia Liu, Jingyi Shi, Lin Yang, Luxiang Cao, Soo Mi Park, Jianmin Cui, Steven O. Marx (2004) Assembly of a Ca2+-dependent BK channel signaling complex by binding to β2 adrenergic receptor. EMBO J 23, 2196-2205. PMCID: 419908 (Download)

3. Lei Hu, Jingyi Shi, Zhongming Ma, Gayathri Krishnamoorthy, Fred Sieling, Guangping Zhang, Frank Horrigan and Jianmin Cui (2003) Participation of the S4 Voltage Sensor in the Mg2+-Dependent Activation of Large Conductance (BK) K+ Channels. Proc. Natl. Acad. Sci., U.S.A. 100, 10488-10493. PMCID: 193588 (Download)

2. Shi, J. Krishnamoorthy, G., Yang, Y., Hu, L., Chaturvedi, N., Harilal, D., Qin, J., and Cui, J. (2002). Mechanism of magnesium activation of calcium-activated potassium channels. Nature 418, 876-880.PMID: 12192410 (Download)

1. Shi, J. and Cui, J. (2001). Intracellular Mg2+ enhances the function of BK-type Ca2+-activated K+ channels. J. Gen. Physiol. 118, 589-605. PMCID: 2233844 [Commentary: J. Gen. Physiol. 118, 583-587]. (Download)

IKs channels

9. Kasimova MA, Zaydman MA, Cui J, Tarek M. (2015) PIP2-dependent coupling is prominent in Kv7.1 due to weakened interactions between S4-S5 and S6. 5:7474. Sci Rep. doi: 10.1038/ srep07474. PMID: 25559286 (Download)

8. Zaydman MA, Kasimova MA, McFarland K, Beller Z, Hou P, Kinser HE, Liang H, Zhang G, Shi J, Tarek M, Cui J. (2014) Domain-domain interactions determine the gating, permeation, pharmacology, and subunit modulation of the IKs ion channel. Elife 3:e03606. doi: 10.7554/eLife.03606. PMID: 25535795 (Download)

7. Li Y, Gao J, Lu Z, Delaloye D, Shi J, Bock K, Cohen IS, and Cui J (2013) Intracellular ATP binding is required to activate the slow activating K+ channel IKs. Proc. Natl. Acad. Sci., U.S.A. 110(47):18922-7. doi: 10.1073/pnas.1315649110 PMID: 24190995 (Download)

6. Zaydman MA, Silva JR, Delaloye K, Li Y, Liang H, Larsson HP, Shi J, and Cui J (2013) Kv7.1 ion channels require PIP2 to couple voltage sensing to pore opening. Proc. Natl. Acad. Sci., U.S.A.  110:13180-13185. PMID: 23151230 (Download)

5. Lee H-C, Rudy Y, Chen P-Y, Sheu S-H, Chang J-G and Cui J (2013) Modulation of KCNQ1 Alternative Splicing Regulates Cardiac IKs Currents and Action Potential Repolarization. HeartRhythm 10:1220-1228. doi: 10.1016/j.hrthm.2013.04.014 PMID: 23608591 (Download)

4. Li, Y., Zaydman, M.A., Wu, D., Shi, J., Guan, M., Virgin-Downey, B.W., and Cui, J. (2011) KCNE1 enhances PIP2 sensitivity of IKs to modulate channel activity. Proc. Natl. Acad. Sci., U.S.A. 108:9095-9100. PMCID: 21576493 (Download)

3. Wu, D., Pan, H., Delaloye, K., and Cui, J. (2010) KCNE1 remodels the voltage sensor of Kv7.1 to modulate channel function. Biophys. J. 99:3599-3608. PMCID: 2998620 (Download)

2. Wu, D., Delaloye, K., Zaydman, M.A., Nekouzadeh, A., Rudy, Y., and Cui, J. (2010) State dependent electrostatic interactions of S4 arginines with E1 in S2 during Kv7.1 activation. J. Gen. Physiol. 135:595- 606. PMCID: 2888051 (Download)

1. Silva, J., Pan, H., Wu, D., Nekouzadeh, A., Decker, K., Cui, J., Baker, N., Sept, D., and Rudy, Y. (2009) Linking Ion-Channel Molecular Dynamics to the Action Potential: Cardiac IKs Simulations. Proc. Natl. Acad. Sci., U.S.A., 106:11102-11106. PMCID: 2700153 (Download)

Ultrasound mediated drug/gene delivery

7. Kubanek J, Shi J, Marsh J, Chen D, Deng C, Cui J. (2016) Ultrasound modulates ion channel currents. Sci Rep. 6:24170. doi: 10.1038/srep24170. PMID: 27112990(Download)

6. Zhou Y., Yang K., Cui J., Ye J.Y., Deng C.X. (2012) Controlled permeation of cell membrane by single bubble acoustic cavitation. J Control Release 157:103-111. PMID: 21945682 (Download)

5. Zhou, Y., Kumon, R.E., Cui, J. and Deng, C.X. (2009) The Size of Sonoporation Pores on the Cell Membrane. Ultrasound in Medicine and Biology 35:1756-1760. PMCID: 2752487 (Download)

4. Zhou, Y., Cui, J. and Deng, C.X. (2008) Dynamics of Sonoporation Correlated with Acoustic Cavitation Activities. Biophys. J., 94:L51-53. PMCID: 2267145 (Download)

3. Zhou, Y., Shi, J., Cui, J. and Deng, C.X. (2007) Effects of extracellular calcium on cell membrane resealing in sonoporation. J. Control. Release. 126:34-43. PMCID: 2270413 (Download)

2. Pan, H., Zhou, Y., Izadnegahdar, O., Cui, J., and Deng, C. X. (2005) Study of Sonoporation Dynamics Affected by Ultrasound Duty Cycle. Ultrasound in Medicine and Biology. 31, 849-856. PMCID: 1182120 (Download)

1. Cheri Deng, Fred Sieling, Hua Pan, and Jianmin Cui (2004) Ultrasound-induced cell membrane porosity. Ultrasound in Medicine and Biology. 30, 519-526. PMID: 15121254 (Download)

Cardiac physiology

4. Li J, Li C, Liang D, Lv F, Yuan T, The E, Ma X, Wu Y, Zhen L, Xie D, Wang S, Liu Y, Huang J, Shi J, Liu Y, Shi D, Xu L, Lin L, Peng L, Cui J, Zhu W, Chen YH. (2016) LRP6 acts as a scaffold protein in cardiac gap junction assembly. Nat Commun. 7:11775. doi: 10.1038/ncomms11775. PMID: 27250245(Download)

3. Varga Z, Zhu W, Schubert AR, Pardieck JL, Krumholz A, Hsu EJ, Zaydman MA, Cui J, Silva JR. (2015) Direct Measurement of Cardiac Na+ Channel Conformations Reveals Molecular Pathologies of Inherited Mutations. Circ Arrhythm Electrophysiol. (5):1228-39. doi: 10.1161/CIRCEP.115.003155. PMID: 26283144(Download)

2. Li, J., Yan B., Huo Z., Liu Y., Xu J., Sun Y., Liu Y., Liang D., Peng L., Zhang Y., Zhou Z.N., Shi J., Cui J., Chen Y.H. (2010) {beta}2- but not {beta}1-adrenoceptor activation modulates intracellular oxygen availability. J. Physiol. 15:2987-2998. PMID: 20547682 (Download)

1. Yiqing Yang, Min Xia, Qingfeng Jin, Said Bendahhou, Jingyi Shi, Yiping Chen, Bo Liang, Jie Lin, Yi Liu, Ban Liu, Qinshu Zhou, Dongwei Zhang, Rong Wang, Ning Ma, Xiaoyan Su, Kaiya Niu, Yan Pei, Wenyuan Xu, Zhaopeng Chen, Haiying Wan, Jianmin Cui, Jacques Barhanin, and Yihan Chen (2004) Identification of a KCNE2 Gain-of-Function Mutation in Familial Atrial Fibrillation. Am. J. Hum. Genet. 75:899-905. PMCID: 1182120 (Download)

Invited reviews

9. Cui J. (2016) Voltage-Dependent Gating: Novel Insights from KCNQ1 Channels. Biophys J. 110(1):14-25. doi: 10.1016/j.bpj.2015.11.023. PMID: 26745405(Download)

8. Yang H, Zhang G, Cui J. (2015) BK channels: multiple sensors, one activation gate. Front Physiol. 6:29 doi: 10.3389/fphys.2015.00029 PMID: 25705194 (Download)

7. Zaydman MA, Cui J. (2014) PIP2 regulation of KCNQ channels: biophysical and molecular mechanisms for lipid modulation of voltage-dependent gating. Front Physiol. 5:195. doi: 10.3389/fphys.2014.00195. PMID: 24904429 (Download)

6. Zaydman, M.A., Silva, J.R., and Cui, J. (2012) Ion channel associated diseases: overview of molecular mechanisms. Chem Rev PMID: 23151230 (Download)

5. Sun, X., Zaydman, M.A., and Cui, J. (2012) Regulation of KV channel voltage-dependent activation by transmembrane β subunits. Front. Pharmacol. 3:63. doi: 10.3389/fphar.2012.00063. PMCID: 3328208 (Download)

4. Cui, J. (2010) BK-type calcium-activated potassium channels: coupling of metal ions and voltage sensing. J. Physiol. 588:4651-4658. PMCID: 3010134 (Download)

3. Lee, U.S. and Cui, J. (2010) BK channel activation: structural and functional insights. Trends in Neurosciences 33:415-423. PMCID: 2929326. (Download)

2. Cui, J. (2010) Reduction of CaV channel activities by Ca2+-CaM: inactivation or deactivation? J. Gen. Physiol. 135:297-301. PMCID: 2847919 (Download)

1. Cui, J., Yang, H., and Lee, U.S. (2009) Molecular mechanisms of activation of Ca2+ activated K+ channels. Cellular and Molecular Life Sciences, 66:852-875. PMCID: 2694844. (Download)

Book Chapter

1. Wu, D. and Cui, J. (2014). Ion channels in the cell membrane: structure, function and modeling. In Comprehensive Biomedical Physics, Anders Brahme eds, Elsevier. (Download)

2. Yang, H. and Cui, J. (2014). BK Channels. In Handbook of Ion Channels, Jie Zheng and Matthew C.  Trudeau ed, Taylor & Francis Books, Inc.


1. Su D, Nian Y, Zhang F, Hu J, Cui J, Zhou M, Yang J, Wang S. Thiazolidine reacts with thioreactive biomolecules. (2017) Free Radic Biol Med. 104:272-279. doi: 10.1016/j.freeradbiomed.2017.01.032. PMID: 28130183(Download)

2. Su D, Zhao H, Hu J, Tang D, Cui J, Zhou M, Yang J, Wang S. (2016) TRPA1 and TRPV1 contribute to iodine antiseptics-associated pain and allergy. EMBO Rep. 17(10):1422-1430. PMID: 27566753(Download)